I’m using ImageJ to quantify reflectance of melanin coloration and extent of melanin coloration (using basic thresholding). I’m new to this plug in and multispectral images. Can I do simple tresholding with multispectral images? If so, how?
Tresholding with multispectral images
jolyon Answered question July 28, 2022
Hi,
I am not sure what your ultimate goal is here? What do you mean by extent? What do you mean by basic/simple thresholding? Please specify as there are a gazillion things that can and can’t be done in the toolbox.
Best,
Cedric
Tresholding with multispectral images
Cedric van den Berg Changed status to publish July 28, 2022
But yes, you can simply create a linear normalised, or cone-catch image and then use ImageJ’s comprehensive thresholding tool. There’s also an adaptive thresholding tool in the toolbox that might be useful if there are low-spatial frequency elements to ignore (e.g. shadows).
Tresholding with multispectral images
jolyon Answered question July 28, 2022